675 .
RUMINOCOCCUS ALBUS MEDIUM
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Selected strain: Blautia wexlerae DSM 19850
This strain uses the standard medium without modifications.
Equipment needed:
Autoclave
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone
(BD-Difco) |
5 | g | ||
|
|
Yeast extract
(BD-Difco) |
2 | g | ||
|
|
Glucose | 3 | g | ||
|
|
Cellobiose | 2 | g | ||
|
|
S I solution 6.0 g/L | 40 | ml | ||
|
|
Mineral solution 2 | 40 | ml | ||
|
|
Resazurin | 1 | mg | ||
|
|
Distilled water | 920 | ml | ||
| 1 Boil and cool under CO2. Under a gentle stream of CO2, add 4.0 g Na2CO3, 1.0 ml fatty acid mixture (see below) and 500 mg L-cysteine x HCl x H2O. | |||||
| 2 Adjust pH to 7.0, distribute into appropriate vessels under N2 and autoclave. | |||||
Mineral solution 2 #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 0.60 | g |
|
|
(NH4)2SO4 | 2.00 | g |
|
|
NaCl | 1.20 | g |
|
|
MgSO4 x 7 H2O | 0.25 | g |
|
|
CaCl2 x 2 H2O | 0.16 | g |
|
|
Distilled water | 100.00 | ml |
Fatty acid mixture #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Isobutyric acid | 10 | ml |
|
|
Isovaleric acid | 10 | ml |
|
|
2-Methylbutyric acid | 10 | ml |
|
|
Distilled water | 70 | ml |