674 .
PYG MEDIUM (F)
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Selected strain: Ruminiclostridium sufflavum DSM 19573
This strain uses the standard medium without modifications.
Equipment needed:
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
S I solution 6.0 g/L | 75.0 | ml | ||
|
|
S II solution | 75.0 | ml | ||
|
|
Trypticase peptone | 10.0 | g | ||
|
|
Yeast extract | 5.0 | g | ||
|
|
Glucose | 10.0 | g | ||
|
|
Sodium resazurin
(w/v, 0.1%) |
1.0 | ml | ||
|
|
Na2CO3
(w/v, 8%) |
2.5 | ml | ||
|
|
L-Cysteine HCl x H2O | 0.3 | g | ||
|
|
Distilled water | 850.0 | ml | ||
| 1 Adjust pH to 7.3. | |||||
S II solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 0.60 | g |
|
|
(NH4)2SO4 | 1.20 | g |
|
|
NaCl | 1.20 | g |
|
|
MgSO4 x 7 H2O | 0.12 | g |
|
|
CaCl2 x 2 H2O | 0.12 | g |
|
|
Distilled water | 100.00 | ml |