JCM 672 . FLEXIBACTER CANADENSIS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
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Medium for Solitalea canadensis DSM 3403

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.1 g 0.1 0.405
KNO3 0.1 g 0.1 0.988
CaCl2 x 2 H2O 0.1 g 0.1 0.68
Sodium glycerophosphate 0.1 g 0.1 0.462
Trace element solution SL-10 1.0 ml - -
Tris(hydroxymethyl)aminomethane 1.0 g 0.999 8.247
Thiamine HCl 1.0 mg 1.0e-3 -
Casamino acids
(BD-Difco)
1.0 g 0.999 -
Glucose 1.0 g 0.999 5.545
Vitamin B12 1.0 �g - -
Distilled water 1000.0 ml - -
1 Adjust pH to 7.5.
Solution occurs in 20 other media (first defined in Medium J433).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%, 7.7 M)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Metadata

Last modified: 24.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
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Associated strains

All strains for this medium: 1
Solitalea canadensis DSM 3403

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tris(hydroxymethyl)aminomethane 0.999 8.247
Glucose 0.999 5.545
Casamino acids 0.999 -
KNO3 0.1 0.988
CaCl2 x 2 H2O 0.1 0.68
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain