669 .
MAGNETOSPIRILLUM MEDIUM (B)
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Selected strain: Paramagnetospirillum magnetotacticum DSM 3856
This strain uses the standard medium without modifications.
Equipment needed:
Autoclave
Final pH:
6.7
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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KH2PO4 | 0.28 | g | ||
|
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NaNO3 | 0.12 | g | ||
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Sodium thioglycolate | 0.05 | g | ||
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Sodium tartrate | 0.37 | g | ||
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Sodium succinate | 0.37 | g | ||
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Sodium acetate | 0.05 | g | ||
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Trace vitamins | 10.00 | ml | ||
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Ferric quinate solution | 2.00 | ml | ||
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Mineral salt solution | 1.00 | ml | ||
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Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 6.75 with NaOH. | |||||
Ferric quinate solution #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeCl3 x 6 H2O | 0.45 | g | ||
|
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Quinic acid | 0.19 | g | ||
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Distilled water | 100.00 | ml | ||
| 1 Dissolve and autoclave at 121C for 15 min. | |||||
| 2 Comment: For strain JCM 21281, good growth can be obtained in semisolid medium (1.0 g/L agar). | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
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Folic acid | 2.0 | mg |
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Pyridoxine hydrochloride | 10.0 | mg |
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
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Nicotinic acid | 5.0 | mg |
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Calcium pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
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p-Aminobenzoic acid | 5.0 | mg |
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Lipoic acid | 5.0 | mg |
|
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Distilled water | 1000.0 | ml |
Mineral salt solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 29.70 | g | ||
|
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Nitrilotriacetic acid | 10.00 | g | ||
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CaCl2 x 2 H2O | 3.34 | g | ||
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FeSO4 x 7 H2O | 99.00 | mg | ||
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Na2MoO4 x 2 H2O | 13.00 | mg | ||
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Metals ''44'' | 50.00 | ml | ||
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Distilled water | 950.00 | ml | ||
| 1 Add nitrilotriacetic acid to 500 ml of distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add the remaining components and distilled water. Readjust pH to 7.2. | |||||