J590
590 .
A-MMX MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Haloplasma contractile DSM 18853
This strain uses the standard medium without modifications.
Main sol. J590 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Synthetic seawater (2 x)
(2 x) |
125.00 | ml | - | - | |
| CaCl2 x 2 H2O | 0.19 | g | 0.19 | 1.292 | |
| NaCl | 113.00 | g | 113 | 1933.61 | |
| KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
| Peptone | 0.20 | g | 0.2 | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Starch solution 5%(w/v) | 100.00 | ml | - | - | |
| Distilled water | 775.00 | ml | - | - | |
| 1 Mix components except Na2S x 9H2O and bubble with N2 for 30 min. Distribute the medium into appropriate culture vessels (e.g., 10 ml medium in the Bellco anaerobic culture tube) under a N2 atmosphere, seal with butyl rubber stoppers. Add Na2S x 9H2O as 5% solution and autoclave. Adjust pH to 6.5 - 7.0, if necessary. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Starch
(soluble) |
5 | g | 50 | - | |
| Distilled water | 100 | ml | - | - | |
| 1 Boil water, then, add starch and mix until dissolves thoroughly. | |||||
Solution occurs in 1
other media (first defined in Medium J590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 55.40 | g | 55.4 | 947.981 |
| MgSO4 x 7 H2O | 14.00 | g | 14 | 56.801 |
| MgCl2 x 6 H2O | 11.00 | g | 11 | 54.106 |
| KCl | 1.30 | g | 1.3 | 17.438 |
| NaBr | 0.20 | g | 0.2 | 1.944 |
| H3BO3 | 0.06 | g | 0.06 | 0.97 |
| SrCl2 x 6 H2O | 0.03 | g | 0.03 | 0.113 |
| KI | 0.10 | mg | 1.0e-4 | 3.9e-4 |
| CaCl2 x 2 H2O | 1.50 | g | 1.5 | 10.203 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Haloplasma contractile DSM 18853
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 119.925 | 2052.11 |
| Starch | 5 | - |
| MgSO4 x 7 H2O | 1.75 | 7.1 |
| MgCl2 x 6 H2O | 1.375 | 6.763 |
| KH2PO4 | 0.5 | 3.674 |
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