MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 556 . METHYLOSOMA DIFFICILE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J556 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 0.100 g 0.1 1.708
MgCl2 x 6 H2O 0.040 g 0.04 0.196
KCl 0.050 g 0.05 0.669
CaCl2 x 2 H2O 0.015 g 0.015 0.102
Na2SO4 0.016 g 0.016 0.112
Distilled water 993.000 ml - -
1 Mix components and autoclave. After cooling, add the following solutions (autoclaved or filter-sterilized) to the medium:
HEPES buffer
(pH 7.0, 2 M) 		5.000 ml - -
1 M Phosphate buffer
(pH 7.2, 1 M) 		0.150 ml - -
KNO3
(0.09 M) 		0.544 ml - -
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
Vitamin solution
(filter-sterilized) 		1.000 ml - -
2 Distribute the culture medium into culture tubes (leave >80% volume as a gas phase) and seal with butyl rubber stoppers.
3 Finally, replace the gas phase with 20% methane and 5% CO2 in air (by volume).

1 M Phosphate buffer #

Solution occurs in 2 other media (first defined in Medium J556).
1 Mix 28 ml of 1 M KH2PO4 and 78 ml of 1 M Na2HPO4 to prepare 100 ml of 1 M phosphate buffer.

Vitamin solution #

1000
Solution occurs in 4 other media (first defined in Medium J556).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
p-Aminobenzoic acid 50 mg 0.05 0.365
Biotin 10 mg 0.01 0.041
Nicotinic acid 100 mg 0.1 0.812
DL-Calcium pantothenate 25 mg 0.025 0.052
Pyridoxine hydrochloride 250 mg 0.25 -
Thiamine HCl 50 mg 0.05 -
Distilled water 1000 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
Edit in Medium builder

Gas composition

  • 5 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.1 1.708
KCl 0.05 0.669
MgCl2 x 6 H2O 0.04 0.196
Na2SO4 0.016 0.112
CaCl2 x 2 H2O 0.015 0.102
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
oxygen microaerophile 1 strain
Show more