50 .
OATMEAL AGAR (ISP-3)
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Selected strain: Streptomyces netropsis DSM 40558
This strain uses the standard medium without modifications.
Equipment needed:
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Oatmeal
(Quaker White Oats) |
20 | g | ||
|
|
Trace salt solution | 1 | ml | ||
|
|
Agar | 18 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Cook or steam 20.0 g of oatmeal in 1.0 L of distilled water for 20 min. Filter through cheesecloth. Bring volume back up to 1.0 L and add 1.0 ml of Trace salt solution. | |||||
| 2 Adjust pH to 7.2. | |||||
Trace salt solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeSO4 x 7 H2O | 0.1 | g |
|
|
MnCl2 x 4 H2O | 0.1 | g |
|
|
ZnSO4 x 7 H2O | 0.1 | g |
|
|
Distilled water | 100.0 | ml |