# J50: OATMEAL AGAR (ISP-3) ## Main sol. J50 20 g Oatmeal (Quaker White Oats) 1 ml **Trace salt solution** 18 g Agar 1000 ml Distilled water 1. Cook or steam 20.0 g of oatmeal in 1.0 L of distilled water for 20 min. Filter through cheesecloth. Bring volume back up to 1.0 L and add 1.0 ml of Trace salt solution. 2. Adjust pH to 7.2. ## Trace salt solution 0.1 g FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 100 ml Distilled water