JCM 455 . ALKALI-REINFORCED CLOSTRIDIAL AGAR

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Equipment needed:

Final pH: 10.0

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ml
Compound Amount Unit
Agar
(Sigma, reinforced clostridial)
51 g
Distilled water 900 ml
1 After autoclaving, aseptically add 100 ml of a 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.