JCM 373 . VY/2-SWS AGAR

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

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Medium for Plesiocystis pacifica DSM 14875

This strain uses the standard medium without modifications.

1 Use Medium No. 341 with the following seawater solution instead of the artificial seawater:
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ferric citrate 0.10 g 0.1 0.408
MgSO4 x 7 H2O 8.00 g 7.992 32.425
CaCl2 x 2 H2O 1.00 g 0.999 6.795
KCl 0.50 g 0.5 6.7
NaHCO3 0.16 g 0.16 1.903
H3BO3 0.02 g 0.02 0.323
KBr 0.08 g 0.08 0.672
SrCl2 x 6 H2O 0.03 g 0.03 0.112
Sodium beta-glycerophosphate 0.01 g 0.01 0.046
Trace element solution 1.00 ml - -
NaCl 20.00 g 19.98 341.889
Distilled water 1000.00 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 100 mg 0.1 -
CoCl2 x 6 H2O 20 mg 0.02 0.154
CuSO4 x 5 H2O 10 mg 0.01 0.04
Na2MoO4 x 2 H2O 10 mg 0.01 0.041
ZnCl2 20 mg 0.02 0.147
LiCl 5 mg 0.005 0.118
SnCl2 x 2 H2O 5 mg 0.005 0.022
H3BO3 10 mg 0.01 0.162
KBr 20 mg 0.02 0.168
KI 20 mg 0.02 0.077
FeNa-EDTA 8 g 8 -
Distilled water 1000 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
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Associated strains

All strains for this medium: 2
Plesiocystis pacifica DSM 14875

No modifications necessary for this strain.

Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains