# J373: VY/2-SWS AGAR ## Main sol. J373 1. Use Medium No. 341 with the following seawater solution instead of the artificial seawater: ## Seawater solution 0.1 g Ferric citrate 8 g MgSO4 x 7 H2O 1 g CaCl2 x 2 H2O 0.5 g KCl 0.16 g NaHCO3 0.02 g H3BO3 0.08 g KBr 0.03 g SrCl2 x 6 H2O 0.01 g Sodium beta-glycerophosphate 1 ml **Trace element solution** 20 g NaCl 1000 ml Distilled water ## Trace element solution 100 mg MnCl2 x 4 H2O 20 mg CoCl2 x 6 H2O 10 mg CuSO4 x 5 H2O 10 mg Na2MoO4 x 2 H2O 20 mg ZnCl2 5 mg LiCl 5 mg SnCl2 x 2 H2O 10 mg H3BO3 20 mg KBr 20 mg KI 8 g FeNa-EDTA 1000 ml Distilled water