341 .
VY/2-ASW AGAR
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast cell paste | 5.0 | g | ||
|
|
Vitamin B12 | 0.5 | mg | ||
|
|
Agar | 15.0 | g | ||
|
|
Sea water
(artificial) |
1000.0 | ml | ||
| 1 Adjust pH to 7.5. Autoclave at 120C for 20 min. Separately sterilize vitamin B12 by filtration, and aseptically add to the medium. | |||||
Yeast cell paste #
| 1 Wash dried yeast (e.g., Yeast from Saccharomyces cerevisiae Type II, Sigma) with distilled water three times. |