J19
19 .
BIFIDOBACTERIUM MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Bifidobacterium pullorum subsp. pullorum DSM 20433
This strain uses the standard medium without modifications.
Main sol. J19 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone , tryptic digest | 10 | g | 9.99 | - | |
|
Beef extract
(BD-Difco) |
5 | g | 4.995 | - | |
|
Yeast extract
(BD-Difco) |
5 | g | 4.995 | - | |
| Glucose | 10 | g | 9.99 | 55.451 | |
| K2HPO4 | 3 | g | 2.997 | 17.207 | |
| Tween 80 | 1 | ml | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 6.8. | |||||
| 2 After sterilization, aseptically add solutions of sodium ascorbate and L-cysteine x HCl to final concentration of 1.0% and 0.05%, respectively. Heat medium not freshly prepared in a steamer for 10 min before addition of the reducing substances. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 25 |
Bifidobacterium pullorum subsp. pullorum DSM 20433
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 9.99 | 55.451 |
| Casein peptone | 9.99 | - |
| Beef extract | 4.995 | - |
| Yeast extract | 4.995 | - |
| K2HPO4 | 2.997 | 17.207 |
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Cultivation metadata from 25 strains
| phyla | 1 | 27 strains |
| oxygen | anaerobe | 25 strains |
| oxygen | facultative anaerobe | 1 strain |
| temperature | mesophilic | 27 strains |