181 .
HORIKOSHI-I MEDIUM
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Equipment needed:
Final pH:
10.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 10.0 | g | ||
|
|
Polypeptone
(Nihon Pharm. Co.) |
5.0 | g | ||
|
|
Yeast extract | 5.0 | g | ||
|
|
K2HPO4 | 1.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
Agar | 15.0 | g | ||
|
|
Distilled water | 900.0 | ml | ||
| 1 After autoclaving, aseptically add 100 ml of a sterile 10% Na2CO3 solution to the medium. Check final pH to be about 10.0. | |||||