174 .
ACIDIANUS INFERNUS MEDIUM (A)
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Selected strain: Metallosphaera sedula DSM 5348
This strain uses the standard medium without modifications.
Equipment needed:
Autoclave
Final gas composition:
10 % CO2
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Final pH:
2.5
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Modified Brock's salt base solution | 1000.0 | ml | ||
|
|
Yeast extract
(BD-Difco) |
0.5 | g | ||
|
|
Sulfur
(powder) |
1.0 | g | ||
| 1 Adjust pH to 2.5 with 10 N H2SO4. Autoclave yeast extract separately and steam sulfur for 3 hr on each of 3 successive days. Cultivate under air enriched with 1-10% CO2. | |||||
Modified Brock's salt base solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
(NH4)2SO4 | 1.30 | g |
|
|
KH2PO4 | 0.28 | g |
|
|
MgSO4 x 7 H2O | 0.25 | g |
|
|
CaCl2 x 2 H2O | 0.07 | g |
|
|
FeCl3 x 6 H2O | 2.00 | mg |
|
|
MnCl2 x 4 H2O | 1.80 | mg |
|
|
Na2B4O7 x 10 H2O | 4.50 | mg |
|
|
ZnSO4 x 7 H2O | 0.22 | mg |
|
|
CuCl2 x 2 H2O | 0.05 | mg |
|
|
Na2MoO4 x 2 H2O | 0.03 | mg |
|
|
VOSO4 x n H2O | 0.03 | mg |
|
|
CoSO4 x 7 H2O | 0.01 | mg |
|
|
Distilled water | 1000.00 | ml |