# J174: ACIDIANUS INFERNUS MEDIUM (A)


## Main sol. J174

1000	ml	**Modified Brock's salt base solution**	
0.5	g	Yeast extract (BD-Difco)	
1	g	Sulfur (powder)	

1. Adjust pH to 2.5 with 10 N H2SO4. Autoclave yeast extract separately and steam sulfur for 3 hr on each of 3 successive days. Cultivate under air enriched with 1-10% CO2.


## Modified Brock's salt base solution

1.3	g	(NH4)2SO4	
0.28	g	KH2PO4	
0.25	g	MgSO4 x 7 H2O	
0.07	g	CaCl2 x 2 H2O	
2	mg	FeCl3 x 6 H2O	
1.8	mg	MnCl2 x 4 H2O	
4.5	mg	Na2B4O7 x 10 H2O	
0.22	mg	ZnSO4 x 7 H2O	
0.05	mg	CuCl2 x 2 H2O	
0.03	mg	Na2MoO4 x 2 H2O	
0.03	mg	VOSO4 x n H2O	
0.01	mg	CoSO4 x 7 H2O	
1000	ml	Distilled water