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JCM 1221 . MODIFIED NITRATE MINERAL SALTS MEDIUM-2

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1221 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 1.00 g 0.984 3.993
CaCl2 x 2 H2O 0.20 g 0.197 1.339
KNO3 0.25 g 0.246 2.434
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
HEPES
(pH 7.0, 1 M) 		2.00 ml - -
Ammonium ferric citrate 4.00 mg 0.004 0.015
Distilled water 1000.00 ml - -
1 Mix components thoroughly and autoclave. After cooling, add the following solutions (autoclaved) to the medium:
Phosphate buffer solution 2.00 ml - -
Vitamin solution 10.00 ml - -
2 Methane: Aseptically distribute the medium into sterile serum bottles (leave &; 80% gas phase by volume) and seal with rubber stoppers. Replace the gas phase with an air-methane gas mixture (75:25, v/v). Cultivate with shaking.
3 Adjust pH to 6.8, if necessary. Cultivate with methane or methanol as a substrate as described below:
4 Methanol: Aseptically distribute the medium into culture vessels (e.g., 20 ml in 60 ml serum bottles), supplement with 4.0 ml per liter filter-sterilized methanol and seal with rubber stoppers.

Phosphate buffer solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.4 g 14 102.876
Na2HPO4 x 2 H2O 3.6 g 36 253.594
Distilled water 100.0 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace vitamins 1000 ml - -
Vitamin B12 20 mg 0.02 0.015
L-Ascorbic acid 5 mg 0.005 0.028
Nicotinamide 5 mg 0.005 0.041

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.984 3.993
KNO3 0.246 2.434
CaCl2 x 2 H2O 0.197 1.339
Na2HPO4 x 2 H2O 0.071 0.499
KH2PO4 0.028 0.203
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Cultivation metadata from 3 strains BacDive

pH 4 - 6 1 strain
phyla 1 3 strains
oxygen aerobe 1 strain
temperature mesophilic 2 strains
temperature thermophilic 1 strain