1130 .
YCFA MEDIUM
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Selected strain: Fusicatenibacter saccharivorans DSM 26062
This strain uses the standard medium without modifications.
Equipment needed:
Filter
Final pH:
7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone
(BD-Difco) |
10.0 | g | ||
|
|
Yeast extract
(BD-Difco) |
2.5 | g | ||
|
|
NaHCO3 | 4.0 | g | ||
|
|
Glucose | 2.0 | g | ||
|
|
Maltose | 2.0 | g | ||
|
|
Cellobiose | 2.0 | g | ||
|
|
L-Cysteine HCl x H2O | 1.0 | g | ||
|
|
Mineral solution I | 150.0 | ml | ||
|
|
Mineral solution II | 150.0 | ml | ||
|
|
Resazurin | 1.0 | mg | ||
|
|
VFA mix | 6.2 | ml | ||
|
|
Hemin solution | 10.0 | ml | ||
|
|
Vitamin solution I | 1.0 | ml | ||
|
|
Distilled water | 660.0 | ml | ||
| 1 Adjust pH to 7.45. | |||||
| 2 After autoclaving, add filter-sterilized 1.0 ml Vitamin solution II (see below). | |||||
Mineral solution II #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 3.0 | g |
|
|
(NH4)2SO4 | 6.0 | g |
|
|
NaCl | 6.0 | g |
|
|
MgSO4 x 7 H2O | 0.6 | g |
|
|
CaCl2 x 2 H2O | 0.6 | g |
|
|
Distilled water | 1000.0 | ml |
VFA mix #
26
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Acetic acid | 17 | ml |
|
|
Propionic acid | 6 | ml |
|
|
n-Valeric acid | 1 | ml |
|
|
iso-Valeric acid | 1 | ml |
|
|
iso-Butyric acid | 1 | ml |
Hemin solution #
25
Vitamin solution I #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Biotin | 5 | mg | ||
|
|
Vitamin B12 | 5 | mg | ||
|
|
p-Aminobenzoic acid | 15 | mg | ||
|
|
Folic acid | 25 | mg | ||
|
|
Pyridoxine hydrochloride | 75 | mg | ||
|
|
Distilled water | 500 | ml | ||
| 1 Vitamin solution II: | |||||
|
|
Thiamine HCl | 25 | mg | ||
|
|
Riboflavin | 25 | mg | ||
|
|
Distilled water | 500 | ml | ||