1014 .
NASU IRON REDUCER MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
6.0
Select a strain (optional):
ml
| 1 Mix components thoroughly and adjust pH to 6.0. Autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and store under a N2 atmosphere): | |||||
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 0.20 | g | ||
|
|
KH2PO4 | 3.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
Yeast extract | 0.50 | g | ||
|
|
Modified Allen's trace metal solution | 1.00 | ml | ||
|
|
Trace vitamins
(*) |
10.00 | ml | ||
|
|
Iron(III) citrate solution | 100.00 | ml | ||
|
|
Distilled water | 900.00 | ml | ||
|
|
L-Cysteine HCl
(25 mM) |
10.00 | ml | ||
|
|
FeCl2
(130 mM) |
10.00 | ml | ||
Modified Allen's trace metal solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MnCl2 x 4 H2O | 1.80 | g |
|
|
Na2B4O7 x 10 H2O | 4.50 | g |
|
|
ZnSO4 x 7 H2O | 0.22 | g |
|
|
CuCl2 x 2 H2O | 0.05 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
VOSO4 x n H2O | 0.03 | g |
|
|
CoSO4 x 7 H2O | 0.01 | g |
|
|
Distilled water | 1000.00 | ml |
Iron(III) citrate solution #
| 1 Mix 2.4 g iron(III) citrate and 90 ml distilled water, and boil until dissolved. Cool to an ambient temperature and adjust pH to 6.0 with 10 N NaOH solution. Fill up to 100 ml with distilled water. |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |