85 .
RPL/0.01%RPA
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Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Medium C82 | fill up to 1000 | ml | ||
|
|
0.01% RPA (Rye Grass-Prescott Agar) | fill up to 1000 | ml | ||
| 1 Biphasic (see separate recipes below and overleaf) | |||||
Mail sol. C82 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Rye grass
(dried, milled) |
1 | g | ||
|
|
Medium C76 | 1000 | ml | ||
| 1 Bring PJ (Mail sol. C 76) to the boil then add rye grass. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes. | |||||
Mail sol. C76 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Stock solution (CaCl2/KCl) | 1 | ml | ||
|
|
K2HPO4
(0.51 g/100 ml stock solution) |
1 | ml | ||
|
|
MgSO4 x 7 H2O
(0.28 g/100 ml stock solution) |
1 | ml | ||
|
|
Deionized water | 1000 | ml | ||
| 1 Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes. | |||||
0.01% RPA (Rye Grass-Prescott Agar) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Rye grass
(dried, milled) |
0.1 | g | ||
|
|
Medium C76 | 1000.0 | ml | ||
|
|
Agar
(Oxoid L11) |
15.0 | g | ||
| 1 Make up 1 litre of Rye GrassPrescott Liquid (RPL - see separate recipe), but use only 0.1 g of rye grass. Mix with 15 g of Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes. | |||||