CCAP 68 . NN

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

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Medium for Acanthamoeba castellanii CCAP1534/2

* Modification: non-pathogenic E. coli food source.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Stock solution 1 5 ml - -
Stock solution 2 5 ml - -
Agar
(Molecular Genetics or Oxoid Agar No. 1)
15 g 15 -
Deionized water 1000 ml - -
1 Add stock solutions to deionized water and make up to 1 litre. In suitable vessels thoroughly disperse the agar in cold amoeba saline solution. Autoclave at 15 psi for 15 minutes.
Solution occurs in 2 other media (first defined in Medium C68).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 12.0 g 24 410.678
MgSO4 x 7 H2O 0.4 g 0.8 3.246
CaCl2 x 6 H2O 0.6 g 1.2 5.478
Solution occurs in 2 other media (first defined in Medium C68).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 14.2 g 28.4 200.058
KH2PO4 13.6 g 27.2 199.875

Metadata

Last modified: 07.10.22
Source: CCAP
Synonyms: Non-Nutrient (Amoeba Saline) Agar
Medium type: Defined medium
Final pH: n.d.
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Associated strains

All strains for this medium: 91
Strains with modifications: 86
Acanthamoeba castellanii CCAP1534/2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 -
Na2HPO4 0.142 1
KH2PO4 0.136 0.999
NaCl 0.12 2.053
CaCl2 x 6 H2O 0.006 0.027
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