1 .
ABM
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Equipment needed:
Final pH:
3.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 0.25 | g | ||
|
|
NaNO3
(75 g/l stock solution) |
10.00 | ml | ||
|
|
CaCl2 x 2 H2O
(2.5 g/l stock solution) |
10.00 | ml | ||
|
|
MgSO4 x 7 H2O
(7.5 g/l stock solution) |
10.00 | ml | ||
|
|
K2HPO4 x 3 H2O
(7.5 g/l stock solution) |
10.00 | ml | ||
|
|
KH2PO4
(17.5 g/l stock solution) |
10.00 | ml | ||
|
|
NaCl
(2.5 g/l stock solution) |
10.00 | ml | ||
|
|
Trace Elements (PIV) | 6.00 | ml | ||
| 1 Once elements are dissolved autoclave at 15 psi for 15 minutes. | |||||
|
|
Vitamin B1 solution | 1.00 | ml | ||
|
|
Vitamin B12 solution | 1.00 | ml | ||
| 2 The stock solutions are those for 3N-BBM+V. Make up to 1 litre with distilled water and adjust the pH to 3.0 with 1M NaOH or 1M HCl. Autoclave at 15 psi for 15 minutes. | |||||
Trace Elements (PIV) #
1000
| 1 Ensure elements are added in the following sequence: | |||||
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 0.750 | g | ||
|
|
FeCl3 x 6 H2O | 0.097 | g | ||
|
|
MnCl2 x 4 H2O | 0.041 | g | ||
|
|
ZnCl2 | 0.005 | g | ||
|
|
CoCl2 x 6 H2O | 0.002 | g | ||
|
|
Na2 MoO4 x 2 H2O | 0.004 | g | ||
| 2 Once elements are dissolved autoclave at 15 psi for 15 minutes. | |||||
Vitamin B1 solution #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Thiamine hydrochloride
(Vitamin B1) |
0.12 | g | ||
| 1 Filter sterile | |||||
Vitamin B12 solution #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Cyanocobalamine
(Vitamin B12) |
0.1 | g | ||
| 1 Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile. | |||||