998 .
MEDIUM FOR ROSEOSPIRA
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Equipment needed:
Autoclave
Filter
Final pH:
6.9
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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KH2PO4 | 0.30 | g | ||
|
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NH4Cl | 0.50 | g | ||
|
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CaCl2 x 2 H2O | 0.05 | g | ||
|
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MgCl2 x 6 H2O | 1.00 | g | ||
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MgSO4 x 7 H2O | 0.50 | g | ||
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NaCl | 20.00 | g | ||
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Trace element solution SL12 | 1.00 | ml | ||
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Yeast extract | 0.50 | g | ||
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Distilled water | 1000.00 | ml | ||
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Vitamins V7 solution (Pfennig et Trüper, 1992) | 1.00 | ml | ||
|
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NaHCO3 | 0.15 | %(w/v) | ||
| 1 Media were autoclaved and cooled under N2/CO2 (90/10, v/v). Vitamin V7 solution (1 ml/l) and NaHCO3, (0.15% w/v) were then aseptically added to the medium. Final pH was adjusted to 6.8-7.0 and the medium was dispensed into sterile 50 ml screw-capped bottles. Organic substrates (5 mM Na-acetate and/or 5 mM di-Na-succinate) were added as substrates before use. In addition, for strains DSM 15114 and AT2115, Na2S x 9 H2O (0.02% w/v) were also added to the medium prior to utilisation. Pure cultures, grown in 50-ml screw-capped bottles were stored at +4°C in the dark for preservation. | |||||
Trace element solution SL12 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Distilled water | 1000.0 | ml | ||
|
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H3BO3 | 300.0 | mg | ||
|
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FeSO4 x 7 H2O | 1.1 | g | ||
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CoCl2 x 6 H2O | 190.0 | mg | ||
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MnCl2 x 2 H2O | 50.0 | mg | ||
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ZnCl2 | 42.0 | mg | ||
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NiCl2 x 6 H2O | 24.0 | mg | ||
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Na2MoO4 x 2 H2O | 18.0 | mg | ||
|
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CuCl2 x 2 H2O | 2.0 | mg | ||
| 1 Sterilized by filtration. | |||||
Vitamins V7 solution (Pfennig et Trüper, 1992) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Distilled water | 1000 | ml | ||
|
|
Biotine | 2 | mg | ||
|
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p-Aminobenzoate | 10 | mg | ||
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Thiamine | 10 | mg | ||
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Pantothenate | 5 | mg | ||
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Pyridoxamine | 50 | mg | ||
|
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Vitamine B12 | 20 | mg | ||
|
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Nicotinate | 20 | mg | ||
| 1 Sterilized by filtration. | |||||