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DSMZ

DSMZ 997 . HNW MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 997 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 1.0 g 1 11.765
NaHCO3 1.0 g 1 11.904
Na2WO4 x 2 H2O 0.1 mg 1.0e-4 3.0e-4
Na2S 0.5 g 0.5 6.324
Wolin's vitamin solution 10.0 ml - -
Synthetic seawater 1000.0 ml - -
NaNO3 100.0 g/l 100 1176.54
NaHCO3 100.0 g/l 100 1190.38
Na2S 100.0 g/l 100 1264.86
1 To prepare the medium, all compounds of DMJ seawater were dissolved in 1 liter of distilled deionized water, and the pH was adjusted to around 7.0 with NaOH at room temperature prior to autoclaving. After autoclaving, filter-sterilized NaNO3 solution (100 g/l), NaHCO3 solution (100 g/l), Na2S solution (100 g/l); pH 7.5) and trace vitamin solution were added. Then the tubes were tightly sealed with butyl rubber stoppers under a gas phase of 80% H2 + 20% CO2 (300 kPa).

Synthetic seawater #

1000
Solution occurs in 1 other media (first defined in Medium 997).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 3000 51334.7
K2HPO4 0.14 g 14 80.379
CaCl2 x 2 H2O 0.14 g 14 95.228
NH4Cl 0.25 g 25 467.377
MgSO4 x 7 H2O 3.40 g 340 1379.44
MgCl2 x 6 H2O 4.18 g 418 2056.04
KCl 0.33 g 33 442.656
NiCl2 x 6 H2O 0.50 mg 0.05 0.386
Na2SeO3 x 5 H2O 0.50 mg 0.05 0.19
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 1 -
Trace mineral solution 10.00 ml - -

Trace mineral solution #

1000
Solution occurs in 1 other media (first defined in Medium 997).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x 2 H2O 0.500 g 0.5 2.673
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -

Wolin's vitamin solution #

1000
Solution occurs in 54 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.05.25
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Filter
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 418 2056.04
Na2S 100 1264.86
NaHCO3 100 1190.38
NaNO3 100 1176.54
KCl 33 442.656
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Cultivation metadata from 2 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
oxygen anaerobe 2 strains
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