993c .
MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE
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Selected strain: Actinopolyspora sp. DSM 45012
This strain uses the standard medium without modifications.
Growth conditions: aerobic
37 °C
pH 7.20
Equipment needed:
Autoclave
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Medium 993 | 1000 | ml | ||
|
|
NaCl | 10 | % | ||
| 1 Prepare medium 993 supplemented with NaCl (10 %). pH should be 7.2. | |||||
| 2 Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish. | |||||
Main sol. 993 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
L-Asparagine
(anhydrous basis) |
1 | g | ||
|
|
Glycerol | 10 | g | ||
|
|
K2HPO4
(anhydrous basis) |
1 | g | ||
|
|
Distilled water | 1000 | ml | ||
|
|
Trace element solution | 1 | ml | ||
|
|
Agar | 20 | g | ||
| 1 The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes. | |||||
| 2 Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish. | |||||
Trace element solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeSO4 x 7 H2O | 0.1 | g |
|
|
MnCl2 x 4 H2O | 0.1 | g |
|
|
ZnSO4 x 7 H2O | 0.1 | g |
|
|
Distilled water | 100.0 | ml |