# 993c: MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE


## Main sol. 993c

1000	ml	**Main sol. 993**	
10	%	NaCl	

1. Prepare medium 993 supplemented with NaCl (10 %). pH should be 7.2.
2. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.


## Main sol. 993

1	g	L-Asparagine (anhydrous basis)	
10	g	Glycerol	
1	g	K2HPO4 (anhydrous basis)	
1000	ml	Distilled water	
1	ml	**Trace element solution**	
20	g	Agar	

1. The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes.
2. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.


## Trace element solution

0.1	g	FeSO4 x 7 H2O	
0.1	g	MnCl2 x 4 H2O	
0.1	g	ZnSO4 x 7 H2O	
100	ml	Distilled water