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DSMZ

DSMZ 992 . BACILLUS FILIFORMIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Salsuginibacillus halophilus DSM 28389

* Modification: Add 7.5 g polypeptone and increase sodium carbonate to 10 g

Growth conditions: 30 °C

Main sol. 992 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 10.0 g 10 -
Sodium citrate 3.0 g 3 11.625
KCl 2.0 g 2 26.828
MgSO4 x 7 H2O 1.0 g 1 4.057
Na2CO3* 10.0 g - -
NaCl 100.0 g 100 1711.16
MnCl2 solution 0.36 g/L 1.0 ml - -
FeSO4 solution 5%(w/v) 1.0 ml - -
Polypeptone* 7.5 g - -
Distilled water 1000.0 ml - -
1 Sodium carbonate and sodium chloride should separately be autoclaved. pH 9.0.

MnCl2 solution 0.36 g/L #

1000
Solution occurs in 1 other media (first defined in Medium 992).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 0.36 g 0.36 1.819
Distilled water 1000.00 ml - -

FeSO4 solution 5%(w/v) #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 50 g 50 179.846
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2
Strains with modifications: 1
Salsuginibacillus halophilus DSM 28389

  7.5 g Polypeptone
  10 g Na2CO3
  Na2CO3

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
NaCl 100 1711.16
Yeast extract 10 -
Polypeptone 7.5 -
Na2CO3 3 28.305
Sodium citrate 3 11.625
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Cultivation metadata from 2 strains BacDive

pH 8 - 10 2 strains
pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 2 strains
oxygen aerobe 1 strain
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