DSMZ 972 . MYCT MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Medium for Lactococcus sp. DSM 10125

This strain uses the standard medium without modifications.


Growth conditions: 26 °C
Equipment needed: Autoclave Wheaton tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 13.60 g 13.6 99.938
(NH4)2SO4 4.00 g 4 30.271
MgCl2 0.20 g 0.2 2.101
Sodium citrate 0.25 g 0.25 0.969
FeSO4 x 7 H2O 25.00 mg 0.025 0.09
MnSO4 x 4 H2O 25.00 mg 0.025 0.112
Yeast extract 3.00 g 3 -
Casein hydrolysate 3.00 g 3 -
Tween 80 1.00 g 1 0.764
Cyclomaltoheptaose
(ß-cyclodextrin)
7.00 g 7 6.168
Distilled water 1000.00 ml - -
1 pH 6.5.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Wheaton tubes
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Associated strains

All strains for this medium: 1
Lactococcus sp. DSM 10125

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 13.6 99.938
Cyclomaltoheptaose 7 6.168
(NH4)2SO4 4 30.271
Casein hydrolysate 3 -
Yeast extract 3 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 1 strain