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DSMZ

DSMZ 946a . CALDITHRIX MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Caldithrix palaeochoryensis DSM 21940

* Modification: Supplement medium after autoclaving with 1.50 g/l NaNO3 added from a sterile anoxic stock solution.

Growth conditions: anaerobic 55 °C

Main sol. 946a #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Synthetic seawater (2 x conc.) 500.00 ml - -
NH4Cl 0.33 g 0.326 6.102
KH2PO4 0.33 g 0.326 2.399
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
Na2CO3 1.00 g 0.989 9.332
Yeast extract
(OXOID) 		3.00 g 2.967 -
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.60 g 0.593 2.471
NaNO3* 1.50 g - -
Distilled water 500.00 ml - -
1 Dissolve ingredients (except carbonate, yeast extract, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere and yeast extract, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of complete medium to 6.8, if necessary.

Modified Wolin's mineral solution #

1000
Solution occurs in 167 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Synthetic seawater (2 x conc.) #

1000
Solution occurs in 3 other media (first defined in Medium 795).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 55.40 g 55.4 947.981
SrCl2 x 6 H2O 0.03 g 0.03 0.113
MgSO4 x 7 H2O 14.00 g 14 56.801
KI 0.10 mg 1.0e-4 3.9e-4
MgCl2 x 6 H2O 11.00 g 11 54.106
Na3-citrate 20.00 mg 0.02 0.077
KCl 1.30 g 1.3 17.438
CaCl2 x 2 H2O 1.50 g 1.5 10.203
NaBr 0.20 g 0.2 1.944
H3BO3 0.06 g 0.06 0.97
Distilled water 1000.00 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 08.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 2
Caldithrix palaeochoryensis DSM 21940

  1.5 g NaNO3

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 5.44 26.759
Yeast extract 2.967 -
NaNO3 1.484 17.456
Na2CO3 0.989 9.332
KCl 0.643 8.624
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Cultivation metadata from 3 strains BacDive

halophily slightly halophilic 1 strain
pH 6 - 8 2 strains
phyla 1 3 strains
oxygen anaerobe 3 strains
oxygen aerotolerant 1 strain
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