942 .
AGAR WITH E. COLI AS FEED
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Equipment needed:
Autoclave
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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CaCl2 x 2 H2O | 1 | g | ||
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Agar | 15 | g | ||
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Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.2, autoclave and pour plates. Grow Escherichia coli (e.g. DSM 498) in liquid culture, collect cells by centrifugation and resuspend them in enough water to receive a thick but ready-to-pipet suspension. Autoclave. Place three separate drops on one CaCl2-agar plate and streak every drop once to receive E. coli lines, about 3 cm long, across the E. coli drops. Inoculate E. coli lines/drops with myxobacteria. | |||||