939 .
METHYLOTROPHIC ARTHROBACTER AND HYPHOMICROBIUM MEDIUM
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* Medium for Hyphomicrobium sulfonivorans DSM 13863
Plus 1%MeOH
Growth conditions: 30 °C
Equipment needed:
Autoclave
Final pH:
7.2 - 7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 x 2 H2O | 7.9 | g | ||
|
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KH2PO4 | 1.5 | g | ||
|
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NH4Cl | 0.8 | g | ||
|
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MgSO4 x 7 H2O | 0.1 | g | ||
|
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Trace elements solution | 10.0 | ml | ||
|
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Distilled water | 1000.0 | ml | ||
|
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Methanol | 10.0 | ml | ||
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Methanol | 1.0 | % | ||
| 1 Final pH 7.2-7.5 | |||||
| 2 After autoclaving add 10 ml methanol to 1 litre of the sterile Mineral Salts Solution. | |||||
Trace elements solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 50.00 | g | ||
|
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ZnSO4 x 7 H2O | 1.00 | g | ||
|
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CaCl2 x 2 H2O | 7.34 | g | ||
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MnCl2 x 4 H2O | 2.50 | g | ||
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CoCl2 x 6 H2O | 0.50 | g | ||
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(NH4)6Mo7O24 | 0.50 | g | ||
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FeSO4 x 7 H2O | 5.00 | g | ||
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CuSO4 x 5 H2O | 0.20 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve the EDTA in about 400 ml of water, then add 9 g NaOH. Dissolve each of the salts individually in about 40-50 ml of water and add them to the EDTA-NaOH solution. Adjust the final pH of the solution to pH 6.0 with 1M NaOH (about 24 ml). Make up to 1 litre with distilled water, store in the dark and do not autoclave the stock solution before it is added to the medium. | |||||