939
939 .
METHYLOTROPHIC ARTHROBACTER AND HYPHOMICROBIUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Hyphomicrobium sulfonivorans DSM 13863
* Modification: Plus 1%MeOH
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C
Main sol. 939 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 x 2 H2O | 7.9 | g | 7.9 | 55.65 | |
| KH2PO4 | 1.5 | g | 1.5 | 11.023 | |
| NH4Cl | 0.8 | g | 0.8 | 14.956 | |
| MgSO4 x 7 H2O | 0.1 | g | 0.1 | 0.406 | |
| Trace elements solution | 10.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| Methanol | 10.0 | ml | 7.92 | 247.176 | |
| Methanol* | 1.0 | % | - | - | |
| 1 Final pH 7.2-7.5 | |||||
| 2 After autoclaving add 10 ml methanol to 1 litre of the sterile Mineral Salts Solution. | |||||
Solution occurs in 1
other media (first defined in Medium 939).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 50.00 | g | 50 | 171.092 | |
| ZnSO4 x 7 H2O | 1.00 | g | 1 | 3.478 | |
| CaCl2 x 2 H2O | 7.34 | g | 7.34 | 49.927 | |
| MnCl2 x 4 H2O | 2.50 | g | 2.5 | 12.632 | |
| CoCl2 x 6 H2O | 0.50 | g | 0.5 | 3.851 | |
| (NH4)6Mo7O24 | 0.50 | g | 0.5 | - | |
| FeSO4 x 7 H2O | 5.00 | g | 5 | 17.985 | |
| CuSO4 x 5 H2O | 0.20 | g | 0.2 | 0.801 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve the EDTA in about 400 ml of water, then add 9 g NaOH. Dissolve each of the salts individually in about 40-50 ml of water and add them to the EDTA-NaOH solution. Adjust the final pH of the solution to pH 6.0 with 1M NaOH (about 24 ml). Make up to 1 litre with distilled water, store in the dark and do not autoclave the stock solution before it is added to the medium. | |||||
Metadata
| Last modified: | 25.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.2 - 7.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Methanol | 7.92 | 247.176 |
| Na2HPO4 x 2 H2O | 7.9 | 55.65 |
| KH2PO4 | 1.5 | 11.023 |
| NH4Cl | 0.8 | 14.956 |
| EDTA | 0.5 | 1.711 |
| Show more | ||
