830b .
MEDIUM R2A FOR DENITRIFYING BACTERIA
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 0.50 | g | ||
|
|
Proteose peptone
(Difco no. 3) |
0.50 | g | ||
|
|
Casamino acids | 0.50 | g | ||
|
|
Glucose | 0.50 | g | ||
|
|
Starch
(soluble) |
0.50 | g | ||
|
|
Na-pyruvate | 0.30 | g | ||
|
|
K2HPO4 | 0.30 | g | ||
|
|
MgSO4 x 7 H2O | 0.05 | g | ||
|
|
KNO3 | 0.50 | g | ||
|
|
Trace element solution SL-10 | 10.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Prepare liquid medium under anaerobic conditions (N2). | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||