802 .
GRACILIBACILLUS MEDIUM
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Equipment needed:
Final pH:
7.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 100.0 | g/l | ||
|
|
MgSO4 x 7 H2O | 20.0 | g/l | ||
|
|
KCl | 2.0 | g/l | ||
|
|
NaBr | 0.1 | g/l | ||
|
|
Yeast extract
(Difco) |
2.0 | g/l | ||
|
|
Trypticase peptone
(BBL) |
2.0 | g/l | ||
|
|
Tris-HCl | 12.0 | g/l | ||
|
|
Trace metals solution | 2.0 | ml | ||
|
|
Distilled water | 998.0 | ml | ||
| 1 Adjust pH to 7.8 (with NaOH) before autoclaving. To the sterile medium, from sterile stock solutions, add 10 ml KH2PO4 (50 g/l), 5 ml CaCl2 (100 g/l), 2 ml FeCl2/MnCl2 (FeCl2 x 4 H2O, 20 g/l and MnCl2 x 4 H2O, 20 g/l), and glucose to a final concentration of 2 g/l. | |||||
|
|
KH2PO4
(50 g/l) |
10.0 | ml | ||
|
|
CaCl2
(100 g/l) |
5.0 | ml | ||
|
|
FeCl2/MnCl2 solution | 2.0 | ml | ||
|
|
Glucose | 2.0 | g | ||
Trace metals solution #
1010
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(32%) |
10 | ml | ||
|
|
FeCl2 x 4 H2O | 2 | g | ||
|
|
CoCl2 x 6 H2O | 250 | mg | ||
|
|
MnCl2 x 4 H2O | 100 | mg | ||
|
|
ZnCl2 | 70 | mg | ||
|
|
H3BO3 | 6 | mg | ||
|
|
Na2MoO4 x 2 H2O | 40 | mg | ||
|
|
NiCl2 x 6 H2O | 70 | mg | ||
|
|
CuCl2 x 2 H2O | 2 | mg | ||
|
|
AlCl3 x 6 H2O | 60 | mg | ||
|
|
Na2WO4 x 2 H2O | 6 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Dissolve salts in the HCl in the oder given above, then make up the solution to 1 litre with distilled water. | |||||