782 .
OM-2 MEDIUM
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Equipment needed:
Autoclave
Final pH:
8.5 - 9.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4-oxalate | 15.00 | g | ||
|
|
NaHCO3 | 10.00 | g | ||
|
|
NaH2PO4 x 2 H2O | 10.00 | g | ||
|
|
NaCl | 0.70 | g | ||
|
|
KCl | 0.57 | g | ||
|
|
MgCl2 x 6 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.01 | g | ||
|
|
Na2S2O3 x 5 H2O | 1.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Agar
, if required
(adjust if required in range 1.5-2.0) |
2.00 | %(w/v) | ||
| 1 pH 6.8-7.0 | |||||
| 2 The first three salts must be dissolved in the indicated sequence. After autoclaving for 20 min at 121°C the pH of the medium shifted to 8.5-9.0. Solid medium contained 1.5 to 2.0% (w/v) of Bacto-Agar (Difco). | |||||