DSMZ 756a . MIXOTROPHIC NITROBACTER MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Nitrobacter vulgaris DSM 10236

This strain uses the standard medium without modifications.


Growth conditions: 7-14 days 28 °C
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.50 g 1.5 -
Peptone 1.50 g 1.5 -
Na-pyruvate 0.55 g 0.55 4.998
Trace element solution 1.00 ml - -
Stock solution 100.00 ml - -
NaNO2 2.00 g 2 28.988
Distilled water 899.00 ml - -
1 Adjust pH to 7.4 with NaOH or KOH.
Solution occurs in 4 other media (first defined in Medium 756).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnSO4 x H2O 33.8 mg 0.034 0.2
H3BO3 49.4 mg 0.049 0.799
ZnSO4 x 7 H2O 43.1 mg 0.043 0.15
(NH4)6Mo7O24 37.1 mg 0.037 -
FeSO4 x 7 H2O 97.3 mg 0.097 0.35
CuSO4 x 5 H2O 25.0 mg 0.025 0.1
Distilled water 1000.0 ml - -
Solution occurs in 4 other media (first defined in Medium 756).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCO3 0.07 g 0.07 0.699
NaCl 5.00 g 5 85.558
MgSO4 x 7 H2O 0.50 g 0.5 2.029
KH2PO4 1.50 g 1.5 11.023
Distilled water 1000.00 ml - -

Metadata

Last modified: 05.12.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
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Associated strains

All strains for this medium: 3
Nitrobacter vulgaris DSM 10236

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaNO2 2 28.988
Yeast extract 1.5 -
Peptone 1.5 -
Na-pyruvate 0.55 4.998
NaCl 0.5 8.556
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature mesophilic 3 strains