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DSMZ

DSMZ 718 . PETROTOGA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Petrotoga miotherma DSM 10691

* Modification: Supplement medium with 1.00 g/l Trypticase peptone and increase amount of yeast extract to 1 g/l.

Growth conditions: anaerobic 55 °C

Main sol. 718 #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.34 g 0.336 4.502
MgCl2 x 6 H2O 4.00 g 3.949 19.423
MgSO4 x 7 H2O 3.45 g 3.406 13.818
NH4Cl 0.25 g 0.247 4.614
CaCl2 x 2 H2O 0.14 g 0.138 0.94
K2HPO4 0.14 g 0.138 0.793
NaCl 18.00 g 17.769 304.055
Modified Wolin's mineral solution 10.00 ml - -
Fe(NH4)2(SO4)2 x 7 H2O
(0.1% w/v) 		2.00 ml 0.002 0.005
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
NaHCO3 1.00 g 0.987 11.751
D-Glucose 5.00 g 4.936 27.397
Yeast extract* 1.00 g - -
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.50 g 0.494 2.055
Trypticase peptone* 1.00 g - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, glucose, yeast extract, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate, adjust pH to 6.5 and dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose, yeast extract, vitamins, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Prior to inoculation adjust pH of complete medium to 6.5 - 6.7, if necessary.

Modified Wolin's mineral solution #

1000
Solution occurs in 167 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 12.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5 - 6.7
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 5
Strains with modifications: 4
Petrotoga miotherma DSM 10691

  1 g Trypticase peptone
  1 g Yeast extract
  Yeast extract

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
D-Glucose 4.936 27.397
MgCl2 x 6 H2O 3.949 19.423
NaHCO3 0.987 11.751
Trypticase peptone 0.987 -
Yeast extract 0.987 -
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Cultivation metadata from 5 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 5 strains
oxygen anaerobe 5 strains
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