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DSMZ

DSMZ 671 . THERMOANAEROBACTER (BA) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Thermoanaerobacter pentosaceus DSM 25963

* Modification: Use 4.00 g/l D-xylose (sterilized separately) instead of cellobiose.

Growth conditions: anaerobic 70 °C

Main sol. 671 #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 0.989 18.492
NaCl 0.10 g 0.099 1.693
MgCl2 x 6 H2O 0.10 g 0.099 0.487
CaCl2 x 2 H2O 0.05 g 0.049 0.336
K2HPO4 x 3 H2O 0.40 g 0.396 2.272
Modified Wolin's mineral solution 10.00 ml - -
Yeast extract 0.75 g 0.742 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
Na2CO3 1.00 g 0.989 9.332
Cellobiose* 4.00 g 3.956 11.559
Cellulose , Avicel SIGMA
optional 		2.00 g 1.978 -
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.25 g 0.247 1.03
D-Xylose* 4.00 g - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, cellobiose, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cellobiose, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of cellobiose and vitamins should be sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary.
2 Note: Some strains can be adapted to cellulose as substrate using 2.00 g/l Avicel microcrystalline cellulose (SIGMA).

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 167 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 30.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 7
Strains with modifications: 2
Thermoanaerobacter pentosaceus DSM 25963

  Cellobiose
  4 g D-Xylose

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
D-Xylose 3.956 26.354
Cellulose
optional 		1.978 -
NH4Cl 0.989 18.492
Na2CO3 0.989 9.332
Yeast extract 0.742 -
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Cultivation metadata from 7 strains BacDive

pH 6 - 8 3 strains
pH 4 - 6 1 strain
pH 8 - 10 1 strain
phyla 2 5 strains
nutrition chemoorganoheterotroph 3 strains
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