MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 665 . SULFOBACILLUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Sulfobacillus thermosulfidooxidans DSM 9293

This strain uses the standard medium without modifications.

Growth conditions: 50 °C

Main sol. 665 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 700 ml - -
Solution B 301 ml - -
Solution C 20 ml - -
1 After autoclaving, combine the three solutions. Medium pH 1.9 - 2.4.

Solution A #

700
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 3.00 g 4.286 32.433
KCl 0.10 g 0.143 1.916
K2HPO4 0.50 g 0.714 4.101
MgSO4 x 7 H2O 0.50 g 0.714 2.898
Ca(NO3)2 0.01 g 0.014 0.087
Distilled water 700.00 ml - -
1 Adjust pH to 2.0 - 2.2 with sulfuric acid.

Solution B #

301
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 44.2 g 146.844 528.187
Distilled water 300.0 ml - -
H2SO4 , 10 N 1.0 ml - -
1 (sterilize by filtration or handle under N2 before autoclaving to prevent oxidation of the iron)

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(1% w/v in water) 		20 ml 0.2 -

Metadata

Last modified: 13.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 1.9 - 2.4
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 6
Sulfobacillus thermosulfidooxidans DSM 9293

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 43.291 155.714
(NH4)2SO4 2.938 22.236
K2HPO4 0.49 2.812
MgSO4 x 7 H2O 0.49 1.987
KCl 0.098 1.314
Show more
CSV
JSON

Cultivation metadata from 6 strains BacDive

pH 0 - 2 1 strain
pH 2 - 4 1 strain
phyla 1 6 strains
oxygen aerobe 1 strain
temperature thermophilic 5 strains