638 .
LACTOBACILLUS MEDIUM III
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* Medium for Limosilactobacillus secaliphilus DSM 17896
Plus aqueous extract of trypsin digested rye-bran, which replaces 90% of the water (see 10919); microaerophilic
Growth conditions: 37 °C
Equipment needed:
Final pH:
6.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone | 10.00 | g | ||
|
|
Meat extract | 5.00 | g | ||
|
|
Yeast extract | 5.00 | g | ||
|
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Glucose | 7.00 | g | ||
|
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Fructose | 7.00 | g | ||
|
|
Maltose | 7.00 | g | ||
|
|
Na-gluconate | 2.00 | g | ||
|
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Na-acetate x 3 H2O | 5.00 | g | ||
|
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(NH4)2 citrate | 2.00 | g | ||
|
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K2HPO4 x 3 H2O | 2.60 | g | ||
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MgSO4 x 7 H2O | 0.10 | g | ||
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MnSO4 x 4 H2O | 0.05 | g | ||
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L-Cysteine HCl x H2O | 0.50 | g | ||
|
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Tween 80 | 1.00 | ml | ||
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Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 6.3 | |||||