623 .
THERMOCOCCUS LITORALIS MEDIUM
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Selected strain: Thermococcus litoralis DSM 5474
This strain uses the standard medium without modifications.
Growth conditions: 83 °C
Equipment needed:
Final gas composition:
100 % N2
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Final pH:
6.5
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Bacto peptone | 5.000 | g | ||
|
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Yeast extract | 1.000 | g | ||
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NaCl | 19.450 | g | ||
|
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MgCl2 x 6 H2O | 12.600 | g | ||
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Na2SO4 | 3.420 | g | ||
|
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CaCl2 x 2 H2O | 2.380 | g | ||
|
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KCl | 0.550 | g | ||
|
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Na2CO3 | 0.610 | g | ||
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KBr | 0.080 | g | ||
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SrCl2 x 6 H2O | 0.057 | g | ||
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Boric acid | 0.022 | g | ||
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Na-meta silicate | 4.000 | mg | ||
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NaF | 2.400 | mg | ||
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KNO3 | 1.600 | mg | ||
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Na2HPO4 | 0.010 | g | ||
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Sulfur | 10.000 | g | ||
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Resazurin | 0.100 | g | ||
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Distilled water | 1000.000 | ml | ||
| 1 Prepare the medium under 100% N2 and adjust final pH to 6.5. The medium, containing sulphur, is sterilized by steaming on three consecutive days for 3 h. Reduce the medium with a sterile Na2S stock solution to give a final concentration of 0.5 g/l. | |||||