# 623: THERMOCOCCUS LITORALIS MEDIUM


## Main sol. 623

5	g	Bacto peptone	
1	g	Yeast extract	
19.45	g	NaCl	
12.6	g	MgCl2 x 6 H2O	
3.42	g	Na2SO4	
2.38	g	CaCl2 x 2 H2O	
0.55	g	KCl	
0.61	g	Na2CO3	
0.08	g	KBr	
0.057	g	SrCl2 x 6 H2O	
0.022	g	Boric acid	
4	mg	Na-meta silicate	
2.4	mg	NaF	
1.6	mg	KNO3	
0.01	g	Na2HPO4	
10	g	Sulfur	
0.1	g	Resazurin	
1000	ml	Distilled water	

1. Prepare the medium under 100% N2 and adjust final pH to 6.5. The medium, containing sulphur, is sterilized by steaming on three consecutive days for 3 h. Reduce the medium with a sterile Na2S stock solution to give a final concentration of 0.5 g/l.