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DSMZ

DSMZ 619 . HYPHOMICROBIUM METHYLOVORUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Hyphomicrobium methylovorum DSM 5458

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 619 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Methanol 10.0 ml 7.92 247.176
(NH4)2HPO4 3.0 g 3 22.717
NaCl 1.0 g 1 17.112
MgSO4 x 7 H2O 0.2 g 0.2 0.811
FeSO4 x 7 H2O 10.0 mg 0.01 0.036
MnSO4 x 1 H2O 5.0 mg 0.005 0.033
Vitamin mixture 5.0 ml - -
Agar 15.0 g 15 44.599
Tap water 1000.0 ml - -
1 Sterilize methanol by filtration, and add to other components sterilized by autoclaving.

Vitamin mixture #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 0.2 mg 2.0e-4 8.2e-4
Calcium pantothenate 40.0 mg 0.04 0.084
Folic acid 0.2 mg 2.0e-4 4.5e-4
Inositol 200.0 mg 0.2 1.11
Niacin 40.0 mg 0.04 0.325
p-Aminobenzoic acid 20.0 mg 0.02 0.146
Pyridoxine hydrochloride 40.0 mg 0.04 0.195
Riboflavin 40.0 mg 0.04 0.106
Thiamine-HCl x 2 H2O 20.0 mg 0.02 0.054
Choline 100.0 mg 0.1 0.96
Vitamin B12 2.0 µg 2.0e-6 1.5e-6
Distilled water 1000.0 ml - -

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Filter
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Associated strains

All strains for this medium: 1
Hyphomicrobium methylovorum DSM 5458

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Methanol 7.92 247.176
(NH4)2HPO4 3 22.717
NaCl 1 17.112
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain