606
606 .
COLBY AND ZATHMAN MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Methylobacillus glycogenes DSM 5685
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 606 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 1.20 | g | 1.2 | 6.89 | |
| KH2PO4 | 0.62 | g | 0.62 | 4.556 | |
| CaCl2 x 6 H2O | 0.05 | g | 0.05 | 0.228 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| NaCl | 0.10 | g | 0.1 | 1.711 | |
| (NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
| Trace elements solution | 1.00 | ml | - | - | |
|
Agar
(purified ) |
15.00 | g | 15 | 44.599 | |
| Methanol | 2.00 | ml | 1.584 | 49.435 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.0. Autoclave at 121°C for 15 min. Cool to 50°C. Add a filter-sterilized solution of methanol to give a final concentration of 0.2%. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CuSO4 x 5 H2O | 5 | mg | 0.005 | 0.02 |
| MnSO4 x 5 H2O | 10 | mg | 0.01 | 0.041 |
| Na2MoO4 x 2 H2O | 10 | mg | 0.01 | 0.041 |
| H3BO3 | 10 | mg | 0.01 | 0.162 |
| ZnSO4 x 7 H2O | 70 | mg | 0.07 | 0.243 |
| CoCl2 x 6 H2O | 5 | mg | 0.005 | 0.039 |
| FeCl3 x 6 H2O | 1 | g | 1 | 3.7 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 6 |
| Strains with modifications: | 1 |
Methylobacillus glycogenes DSM 5685
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Methanol | 1.584 | 49.435 |
| K2HPO4 | 1.2 | 6.89 |
| KH2PO4 | 0.62 | 4.556 |
| (NH4)2SO4 | 0.5 | 3.784 |
| Show more | ||
