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598 .
HALOVIBRIO VARIABILIS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Halomonas utahensis DSM 3051
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 598 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 95.0 | g | 95 | 1625.6 | |
| MgSO4 x 7 H2O | 81.0 | g | 81 | 328.632 | |
| KCl | 1.0 | g | 1 | 13.414 | |
| Yeast extract | 7.5 | g | 7.5 | - | |
| Proteose peptone | 2.5 | g | 2.5 | - | |
| Trace element solution SL-4 | 10.0 | ml | - | - | |
| Vitamin solution | 10.0 | ml | - | - | |
| Distilled water | 980.0 | ml | - | - | |
| 1 pH 7.5 | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 0.20 | mg | 2.0e-4 | 8.2e-4 |
| Folic acid | 0.20 | mg | 2.0e-4 | 4.5e-4 |
| Pyridoxine hydrochloride | 1.00 | mg | 1.0e-3 | 0.005 |
| Thiamine-HCl x 2 H2O | 0.50 | mg | 5.0e-4 | 0.001 |
| Riboflavin | 0.50 | mg | 5.0e-4 | 0.001 |
| Nicotinic acid | 0.50 | mg | 5.0e-4 | 0.004 |
| Pantothenic acid | 0.50 | mg | 5.0e-4 | 0.002 |
| Vitamin B12 | 0.01 | mg | 1.0e-5 | 7.4e-6 |
| p-Aminobenzoic acid | 0.50 | mg | 5.0e-4 | 0.004 |
| Lipoic acid | 0.50 | mg | 5.0e-4 | 0.002 |
| Distilled water | 1000.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA | 0.50 | g | 0.5 | 1.487 | |
| FeSO4 x 7 H2O | 0.20 | g | 0.2 | 0.719 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
| H3BO3 | 0.30 | g | 0.3 | 4.851 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Halomonas utahensis DSM 3051
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 95 | 1625.6 |
| MgSO4 x 7 H2O | 81 | 328.632 |
| Yeast extract | 7.5 | - |
| Proteose peptone | 2.5 | - |
| KCl | 1 | 13.414 |
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