593 .
10% MH MEDIUM
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Selected strain: Salinicoccus hispanicus DSM 5352
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 81.000 | g | ||
|
|
MgCl2 | 7.000 | g | ||
|
|
MgSO4 | 9.600 | g | ||
|
|
CaCl2 | 0.360 | g | ||
|
|
KCl | 2.000 | g | ||
|
|
NaHCO3 | 0.060 | g | ||
|
|
NaBr | 0.026 | g | ||
|
|
Proteose peptone no. 3
(Difco) |
5.000 | g | ||
|
|
Yeast extract
(Difco) |
10.000 | g | ||
|
|
Glucose | 1.000 | g | ||
|
|
Distilled water | 1000.000 | ml | ||
|
|
Agar , for solid medium | 15.000 | g/l | ||
| 1 Final pH 7.5 | |||||
| 2 NaHCO3 is added from a sterilized stock solution when the medium has cooled. Agar is added at a concentration of 15.0 g/l for solid media. | |||||