# 593: 10% MH MEDIUM


## Main sol. 593

81	g	NaCl	
7	g	MgCl2	
9.6	g	MgSO4	
0.36	g	CaCl2	
2	g	KCl	
0.06	g	NaHCO3	
0.026	g	NaBr	
5	g	Proteose peptone no. 3 (Difco)	
10	g	Yeast extract (Difco)	
1	g	Glucose	
1000	ml	Distilled water	
15	g/l	Agar, for solid medium	

1. Final pH 7.5
2. NaHCO3 is added from a sterilized stock solution when the medium has cooled. Agar is added at a concentration of 15.0 g/l for solid media.