549 .
CM + YE MEDIUM
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Selected strain: Actinopolyspora mzabensis DSM 45460
This strain uses the standard medium without modifications.
Growth conditions: aerobic
28 °C
pH 7.20
Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casamino acids
(vitamin free, Difco) |
7.5 | g | ||
|
|
Yeast extract | 10.0 | g | ||
|
|
MgSO4 x 7 H2O | 20.0 | g | ||
|
|
Na3-citrate | 3.0 | g | ||
|
|
KCl | 2.0 | g | ||
|
|
NaCl | 200.0 | g | ||
|
|
Fe solution | 1.0 | ml | ||
|
|
Agar | 15.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 pH 7.4 (NaOH) | |||||
Fe solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeSO4 x 7 H2O | 10 | g | ||
|
|
HCl
(0.001 M) |
1000 | ml | ||
| 1 Prepare 4.98% FeSO4 x 7 H2O (= 10 g Fe in 1l 0.001 M HCl) | |||||