# 549: CM + YE MEDIUM


## Main sol. 549

7.5	g	Casamino acids (vitamin free, Difco)	
10	g	Yeast extract	
20	g	MgSO4 x 7 H2O	
3	g	Na3-citrate	
2	g	KCl	
200	g	NaCl	
1	ml	**Fe solution**	
15	g	Agar	
1000	ml	Distilled water	

1. pH 7.4 (NaOH)


## Fe solution

10	g	FeSO4 x 7 H2O	
1000	ml	HCl (0.001 M)	

1. Prepare 4.98% FeSO4 x 7 H2O (= 10 g Fe in 1l 0.001 M HCl)