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DSMZ

DSMZ 547 . ISP MEDIUM 4

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Streptomyces synnematoformans DSM 41902

* Modification: Plus 3% NaCl

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: aerobic 28 °C pH 7.20

Main sol. 547 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution I 500 ml - -
Solution II 501 ml - -
Agar 20 g 19.98 59.406
NaCl* 30 g/l - -
1 The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min.

Solution I #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Starch
(soluble) 		10 g 20 -
Distilled water 500 ml - -
1 Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml.

Solution II #

501
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCO3 2 g 3.992 39.884
K2HPO4
(anhydrous) 		1 g 1.996 11.46
MgSO4 x 7 H2O 1 g 1.996 8.098
NaCl 1 g 1.996 34.155
(NH4)2SO4 2 g 3.992 30.211
Distilled water 500 ml - -
Trace salt solution 1 ml - -

Trace salt solution #

100
Solution occurs in 6 other media (first defined in Medium 547).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.1 g 1 3.597
MnCl2 x 4 H2O 0.1 g 1 5.053
ZnSO4 x 7 H2O 0.1 g 1 3.478
Distilled water 100.0 ml - -

Metadata

Last modified: 19.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
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Associated strains

All strains for this medium: 5
Strains with modifications: 1
Streptomyces synnematoformans DSM 41902

  30.0 g/l NaCl

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
NaCl 30 513.347
Agar 19.98 59.406
Starch 9.99 -
CaCO3 1.998 19.962
(NH4)2SO4 1.998 15.12
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Cultivation metadata from 5 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 5 strains
oxygen aerobe 2 strains
oxygen obligate aerobe 2 strains
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